CRISPR interference (CRISPRi) allows researchers to down-regulate specific gene function by blocking transcription, without editing the DNA. Visit our CRISPRi applications page to learn more.
The Horizon CRISPRmod CRISPRi system requires two components to operate: a gene-specific CRISPRi guide RNA and a catalytically deactivated Cas9 (dCas9) fused to transcriptional repressor domains (SALL1 and SDS3). Our CRISPRi reagents provide a straightforward, efficient set of tools to study a gene’s function via transcriptional repression.
CRISPRi guide RNA
Algorithm-designed lentiviral and synthetic single guide RNA (sgRNA) specifically target immediately downstream of the gene's transcriptional start site (TSS).
The most rapid CRISPRi system, with repression within 24 hours post-transfection. Synthetic RNAs are modified for nuclease resistance and available as pooled or individual reagents.
An ideal delivery method for CRISPRi in difficult-to-transfect cells, or when longer term repression is desired.
Nuclease-deactivated Cas9, fused to our proprietary transcriptional repressor domains SALL1 and SDS3.
Co-transfect with synthetic sgRNA using Dharmafect transfection reagents for transient CRISPRi in lentiviral-free workflows.
Create a stable dCas9 expressing cell line, ready for CRISPRi studies.
CRISPRi assay considerations for reagent selection
There are many options and considerations for CRISPRi experimental conditions. Generally, users achieve the most robust knockdown when working with a stable population of dCas9-SALL1-SDS3 expressing cells. For extended timepoint assays (more than 120 hours), many choose lentiviral sgRNA. For short-term assays (less than 120 hours), synthetic sgRNA gives more robust gene repression than expressed guide RNAs.
|CRISPRi dCas9-SALL1-SDS3 source||Guide RNA format||Delivery method||Benefits & Recommended uses|
transduction & selection for CRISPRi dCas9 stable cells
|CRISPRi synthetic sgRNA||Transfection or electroporation||
|CRISPRi lentiviral sgRNA particles||Transduction||
|CRISPRi dCas9-SALL1-SDS3 mRNA||CRISPRi synthetic sgRNA||Co-transfection or electroporation||