DharmaFECT transfection reagents
For efficient siRNA & CRISPR guide RNA delivery
Optimized solutions for RNA transfection into a wide range of cell types for successful gene editing experiments.
Ensure proper RNA delivery with DharmaFECT™ low-toxicity transfection reagents.
Ancillary buffers & media
High quality buffers & media
Complete your experiment with all necessary ancillary reagents, including buffers, water, and specialized cell culture media.
Viral packaging
Generate your own lentiviral particles
Efficiently generate replication-incompetent lentiviral particles to deliver and express an shRNA or ORF construct into mammalian cells.
Co-transfecting Cas9 mRNA & synthetic CRISPR guide RNA
Quick protocol for CRISPR gene knockout
An abbreviated protocol for transfecting Edit-R™ synthetic guide RNA and Cas9 mRNA into cultured mammalian cells using DharmaFECT™ Duo transfection reagent
Co-transfecting Cas9, guide RNA & HDR donor template
Quick protocol for CRISPR based HDR mediated knock-in
A protocol for transfecting Edit-R™ synthetic guide RNA and Cas9 mRNA with an HDR donor template into cultured mammalian cells using DharmaFECT™ Duo transfection reagent
Pro tips for a successful transfection
Ensure successful RNA deliver every time
Read our top suggestions for transfection success! See how to select the right reagents for your needs and optimize conditions to achieve the best possible results.
Transfecting CRISPR guide RNA into stable Cas9 expressing cells
A straightforward workflow for CRISPR gene knockout
This application note describes optimizing the reverse transfection of synthetic guide RNA into a Cas9-expressing cell line using DharmaFECT™ transfection reagents