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Dharmacon™ RNAi Controls

Critical to any gene silencing or microRNA modulation experiment, RNAi controls enable accurate interpretation for reliable, reproducible results.

  • Positive control
    Validated RNAi reagents to optimize and monitor efficiency of delivery across experimental replicates, conditions or cell types.
  • Non-targeting negative control
    An RNAi reagent with a non-targeting sequence to provide a baseline reference for target-specific knockdown and distinguish sequence-specific silencing from non-specific effects.
  • RISC-Free™, Empty Vector or mock transfection
    A specialized control or experimental condition that lacks a fully functional RNAi component to detect non-RNAi cellular effects (toxicity or gene expression changes) caused by the transfection or transduction.
  • Fluorescent Controls
    Fluorescent dye-labeled controls, including novel nuclear-localizing transfection Indicators permit correlation of signal intensity with optimal siRNA/microRNA transfection conditions.
siRNA Controls
  • ON-TARGETplus 2.0 Controls

    Validated positive and non-targeting negative controls featuring proprietary synthetic modifications for superior specificity, and optimal for use with ON-TARGETplus 2.0 siRNAs.

  • Accell 2.0 controls

    Optimize your Accell 2.0 siRNA delivery conditions with confidence using validated fluorescent or unlabeled positive and negative controls, available as ready-to-use kits.

  • ON-TARGETplus Controls

    Validated positive controls and non-targeting negative controls utilizing patented modifications for optimal specificity. Available as pools or single siRNAs.

  • Accell Controls

    Effectively optimize Accell siRNA delivery conditions with fluorescent or unlabeled positive and negative controls. Kits of validated controls available.

  • siGENOME Controls

    A robust selection of validated positive, negative, and novel RISC-Free® controls to effectively assess all aspects of RNAi experiments.

  • Lincode Controls

    Endogenous lncRNA positive control and non-targeting control siRNA and pool reagents for high-confidence experiments.

  • siGLO Controls

    Fluorescent controls are effective tools for visualizing transfection success. Choose from positive control siRNAs or our novel transfection indicators.

  • Reporter Genes, siSTABLE and other Controls

    Choose from siSTABLE-modified controls, siRNA for silencing reporter genes (GFP, Luciferase, etc.) or other experimental needs.

shRNA Controls
microRNA Controls
Accell 2.0 Control siRNA Kits

A comprehensive set of controls designed to assess and optimize Accell 2.0 siRNA performance in your cell type, supporting confident interpretation of gene silencing results. These controls are matched with Accell 2.0 siRNA designs, helping maintain consistency with your experimental reagents.

Each kit contains:

  • Accell 2.0 GAPD Control siRNA (Human, Mouse)
  • Accell 2.0 Cyclophilin B Control siRNA (Human, Mouse)
  • Accell 2.0 non-targeting siRNA #1
  • Choice of Green or Red Accell 2.0 Non-targeting siRNA (for uptake visualization)
  • 5X siRNA Buffer (optimized for Accell 2.0 delivery)
  • Accell siRNA Delivery Media

Designed to support optimization and experimental control, these kits provide a strong foundation for transfection-free gene silencing workflows in difficult-to-transfect cells

siGENOME Control Kits

A comprehensive array of siRNA control reagents for use in any siRNA experiment.

Each siGENOME Controls Basic Kit contains 5nmol each of:

  • siGENOME Lamin A/C Control siRNA (Human, Mouse, or Rat)
  • siGENOME Cyclophilin B Control siRNA (Human, Mouse, or Rat)
  • siGENOME Non-targeting siRNA #2
  • siGENOME Non-targeting siRNA #5
  • siGENOME RISC-Free Control siRNA
  • siGLO Green Transfection Indicator
  • siGENOME Non-targeting siRNA Pool #2

Each siGENOME Controls Complete Kit additionally contains:

  • Three additional siGLO control siRNAs
  • TOX Transfection Control

 Species
siGENOME Controls Basic Kit Human, Mouse, Rat
siGENOME Controls Complete Kit Human, Mouse, Rat
Which RNAi control is best for you?

Use the guide below to help determine which RNAi control is best for your research needs.



 Product Line Endogenous Positive Control Exogenous Positive Control Negative Control Fluorescent Label Control Kits
siRNA siGenome siRNA Lamin A/C, PPIB, GAPD
Non-targeting, RISC-Free

ON-TARGETplus 2.0 siRNA PPIB, GAPD
Non-targeting

ON-TARGETplus siRNA PPIB, GAPD
Non-targeting

 Accell 2.0 siRNA PPIB, GAPD   eGFP  Non-targeting

Accell siRNA PPIB, GAPD eGFP Non-targeting

siSTABLE siRNA PPIB, GAPD
Non-targeting

siGLO siRNA Lamin A/C, PPIB
RISC-Free

siGLO Transfection Indicators




Reporter gene controls
RLuc,FLuc,GPF,b-glal, GL2 and GL3 Luciferase


Lincode siRNA GAS5
Non-targeting

shRNA SMARTvector Lentiviral shRNA PPIB, GAPD Luciferase Non-targeting, empty vector

SMARTvector Inducible shRNA PPIB, GAPD
Non-targeting

GIPZ Lentiviral shRNA EG5,GAPD
Non-targeting, empty vector

TRIPZ Inducible Lentiviral shRNA GAPD
Non-targeting, empty vector

TRC Lentiviral shRNA
eGFP Empty vector

microRNA shMIMIC Lentiviral microRNA PPIB, GAPD
Non-targeting, empty vector

shMIMIC Inducible microRNA PPIB, GAPD
on-targeting

miRIDIAN microRNA Mimics miR-122,3' UTR controls
Negative Control

miRIDIAN microRNA Hairpin Inhibitors miR-16
Negative Control