CRISPR Guide RNA Designer

For use with S.pyogenes Cas9 (synthetic sgRNA, crRNA, or lentiviral sgRNA)
Enter a 17-23 nt target sequence. Repeat region will be appended automatically
For use with any Cas9 species
crRNA: Enter 35-45 nt consisting of target sequence and repeat region
Synthetic sgRNA: Enter 97-103 nt consisting of target sequence, repeat region, linker, and tracrRNA