Human ON-TARGETplus 2.0 siRNA Libraries
Generate hits that stand up to validation. ON-TARGETplus™ 2.0 siRNA libraries advance high throughput functional gene perturbation with highly potent and on-target gene knockdown through a combination of bioinformatic, formulation, and chemical design choices.
ON-TARGETplus 2.0 is built using the next-generation SMARTselection™ siRNA design algorithm that accounts for the diverse modern transcriptome. This is combined with the proprietary ON-TARGETplus dual-strand chemical modification strategy and optionally formulated in the unique siRNA SMARTpool™ format. The combination of these strategies results in multiple mechanisms to minimize off-target effects and ultimately generate high confidence successful hits in screening.
These siRNA libraries are available in collections spanning the human genome as well as key gene families and druggable collections; and are available in siRNA SMARTpools and set of four individual siRNA formats.
ON-TARGETplus™ 2.0 siRNA libraries
Gain confidence in your screening results by using reagents that reflect biologically relevant genomic data to reduce false positives/negatives. The next-generation RNAi SMARTselection algorithm and sequence curation method used in ON-TARGETplus 2.0 siRNA strives for optimal transcriptomic coverage and further reduced off-targets by considering current gene annotations. Combined with over two decades of experience in siRNA bioinformatic design and RNA chemical synthesis ON-TARGETplus 2.0 siRNA provides highly potent and on-target gene knockdown.
These collections provide a highly diverse set of reagents allowing interrogation of hundreds or thousands of genes with collections spanning the whole human genome, the druggable genome, and commonly studied gene families, providing flexible options for both focused and broad discovery in screening.
Flexibility in arrayed plating format and reagent formulation with siRNA pools or arrays of individual unique siRNA designs supports flexible screening approaches. Ultimately these collections provide the basis to support various single-well analytical techniques such as high content imaging, morphological profiling, reporter-based assays, and high throughput transcriptomics, supporting clear and interpretable data for high throughput screening using genetic perturbation with RNAi.
Highlights
See supporting data tab for data figures supporting the highlights below
- NEW in ON-TARGETplus 2.0 - Modernized transcriptome alignment & re-design: siRNAs are re-annotated and re-designed according to the latest transcriptome data for improved specificity. siRNAs are then curated so that individual siRNAs and SMARTpool siRNAs maximize transcript isoform coverage and ultimately minimize unintended off-targeting by avoiding newly annotated genes or changes in genomic coordinates.
- The proprietary dual-strand ON- TARGETplus chemical modification strategy employs two distinct mechanisms to reduce off- target effects
- Sense strand modification limits RISC loading and promotes selective antisense/active strand loading into the RISC.
- Antisense strand seed region is chemically modified to destabilize microRNA-like off-target activity and enhance target specificity.
- Available as siRNA SMARTpool reagents which minimize individual siRNA seed-mediated off-target signatures through dilution while maintaining potent on-target knockdown.
- Bioinformatic design excludes known seed regions that occur frequently in the genome and selects for low-frequency designs for siRNA to reduce off-targeting.
- Available in 96 or 384-well plate formats. Echo acoustic dispensing compatible plates available upon request.
- Arrayed with space to allow spiking of reagents for controls on each plate.
- Also available as siRNA cherry pick libraries for custom gene selection and hit follow-up efforts.
Available pre-defined collections and approximate gene counts:
Please note, the whole genome library contains the Druggable Genome collection which contains 8 discrete sub-collections, and all remaining protein coding genes annotated in the relevant reference genome.
| Human ON-TARGETplus 2.0 siRNA libraries | Approximate number of genes |
|---|---|
| Whole Genome | 19,600 |
| Druggable Genome | 7,830 |
| GPCRs | 470 |
| Ion Channels | 420 |
| Phosphatases | 310 |
| Proteases | 690 |
| Protein Kinases | 760 |
| Ubiquitin Enzymes | 760 |
| Transcription Factors | 1,570 |
| Drug Targets | 3,300 |
| Ubiquitin Conjugation Subset 1 | 90 |
| Ubiquitin Conjugation Subset 2 | 110 |
| Ubiquitin Conjugation Subset 3 | 390 |
| Apoptosis | 550 |
| Cell Cycle Regulation | 170 |
| Cytokine Receptors | 140 |
| Deubiquitinating Enzymes | 110 |
| DNA Damage Response | 240 |
| Epigenetics | 850 |
| Membrane Trafficking | 140 |
| Nuclear Receptors | 50 |
| Tyrosine Kinases | 90 |
"The light blue shading collections represents part of druggable genome"
Library gene lists
For a complete list of target genes in each siRNA library, please contact Scientific Support.
