For TurboGFP tagged HAP1 Cell Lines, what strategy is used to tag the cells?
We insert the desired tag using a homology-independent strategy we published in Nature Communications. In this strategy the target gene is cleaved at the site where the tag will be integrated using Cas9 programmed with a gRNA. The tag is the inserted via non-homologous end joining at the site where the gRNA cleaved. For more information, please see: A generic strategy for CRISPR-Cas9-mediated gene tagging doi: 10. 1038/ncomms10237