COVID-19 impacts us all in unexpected ways - If you're still in the lab or working at home please let us know how we can help - Contact Us

Do I need to use tracrRNA with MAD7 nuclease?

tracrRNA is not needed for MAD7. The 42-mer crRNA is all that is required to guide the MAD7 nuclease to its target sequence. MAD7 is a Cas12a/TypeV-A nuclease, meaning it uses only a crRNA and no tracrRNA