How do I calculate how much tracrRNA and Tris buffer I will need for my crRNA library?

Since crRNA and tracrRNA are used in equimolar amounts, be sure to purchase enough tracrRNA to complete experiments with all gene-targeting and control crRNAs. For example: For 0. 1 nmol crRNA library: 0. 1 nmol x 100 genes = 10 nmols of tracrRNA For 0. 25 nmol library: 0. 25 nmol x 100 genes = 25 nmols of tracrRNA For 0. 5 nmol library: 0. 5 nmol x 100 genes = 50 nmols of tracrRNA