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How do I test guide RNA activity?

Home> FAQ> How do I test guide RNA activity?How do I test guide RNA activity?Given the time intensive nature of gene engineering, the relatively straightforward and quick (<1 week) process of gRNA validation can save weeks of cell culture and hours of bench time. Further to this, a clear idea of gRNA activity will provide insights into how many clones need to be screened to identify a positive. There are now a variety of methodologies available to scientists looking to compare and validate guide RNA activity. Six of the most commonly used approaches are: Mismatch cleavage assaySequence trace decomposition analysisIndel Detection by Amplicon Analysis (IDAA)Digital PCRImmunofluorescence analysisClonal analysis