What quality control testing is performed for the synthetic tracrRNA and crRNAs?

The Edit-R tracrRNA is purified by HPLC and evaluated by MALDI-TOF mass spectrometry and HPLC to confirm the length and purity. Synthesized crRNAs are evaluated with MALDI-TOF mass spectrometry. All synthetic RNAs are quantified by readings at A260 using a spectrophotometer.