How can I get information on any IMAGE produced cDNA clone?
Below is an example of how to get information on any IMAGE cDNA clone. Put the catalog number, clone ID, or accession number associated with the clone you are interested in into the Search on our website. An example is catalog number MHS6278-202828338, clone ID 3506845, accession number BC000784. Click this link to query for this clone on our website: http: //dharmacon. horizondiscovery. com/search/?term=3506845Click on the orange "+" to the left of the search result. For general cloning details, click on the appropriate tab in the query results to see details, sequence, documents, etc. Things to keep in mind: IMAGE clones were created using OligoDT priming and then directionally cloned into the various vectors using adaptors and ligators containing the restriction sites listed for each clone. These sites were used to clone the insert INTO the vector and we cannot make any guarantees that they will work to clone the insert OUT of the vector. You should always check the sequence of your insert for cut sites to ensure that you can use the restriction sites listed. To do this, go back to the search result for your cDNA clone using our Gene search and click on the BC accession number associated with your clone. The BC accession number is the sequence of the insert for this clone. Clicking on the BC accession from our website will take you to the NCBI record. Link to the BC accession number: http: //www. ncbi. nlm. nih. gov/entrez/viewer. fcgi?db=nuccore&id=34783885To check for restriction sites in the sequence of your insert, copy the insert sequence and then using a tool like Restriction Mapper (www. restrictionmapper. org), you can analyze the sequence for all or select restriction sites. To do this, go to the site above, paste your copied insert sequence into the box, select your enzymes, and then hit "map sites". This will show you the location of the requested restriction enzyme cut sites in your sequence. Another thing to keep in mind, is that the vector maps as posted on our website have been run through a general program that sometimes does not display all of the relevant cut sites for the clone. We have alternate maps available by request, or you can go back to the IMAGE website, click on "resources" and then "vector" to view maps and sequences of all vector used in the IMAGE collection. *IMAGE project status = terminated -- image. llnl. gov is no longer available - The clone collections have been transfered to HAIB. The new IMAGE web site is http: //www. imageconsortium. org/For specific information that is not included on our website, you can look up the details submitted to IMAGE by the lab that created this clone. From the cloning details on our website, in the field labeled "Detailed Description", copy the library name. For this clone it is NIH_MGC_14. Go to the IMAGE website (http: //www. imageconsortium. org/) click on the word "resources", and then the species for your clone, in this case "human". Now search for the library name on the page. In this case it is line 275. Click on the library name to view the information reported by the lab that created this clone. This is the most accurate and complete source of information for any IMAGE clone. If the info is not there it is not available.