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Can RPMI media be substituted for the DMEM media listed in the culturing protocol for the HEK293T cells?

We do not recommend that you use the RPMI media unless for someexperimental reason you have to use it. The two medias do not containthe same components and our HEK293T cells areacclimated to the DMEM media listed in the culturing protocol in theTrans-Lentiviral Packaging System manual.