What is the best method to transfect miRIDIAN microRNA Mimics and Inhibitors?
The miRIDIAN microRNA Mimics and Inhibitors can be delivered using standard lipid-mediated or electroporation delivery methods. The optimal methods and conditions for delivery will depend on the specific cell line or cell type that you are using. They should be similar to those for the delivery of other small nucleic acids (e.g. single-stranded oligos or siRNAs) into the same cell line. DharmaFECT Transfection Reagents can be used to transfect mimics and inhibitors. Cell line-specific transfection conditions are available in the DharmaFECT General Transfection protocol (see Table 2). Concentration and time curves should always be conducted. Suggested concentrations include 100 nM, 50 nM,10 nM, 2 nM, 0.4 nM, while suggested time points are 24, 48 and 72 hours. However, ideal concentrations must be empirically determined for each microRNA mimic or inhibitor tested in each cell type, as for siRNA experimentation.