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How are the SMARTvector lentiviral particles manufactured?

SMARTchoice shRNA Lentiviral particles are manufactured using the Dharmacon Trans-Lentiviral shRNA Packaging System which has been optimized for achieving high viral titers as well as premium biosafety. The SMARTvector shRNA vector construct and the Trans-Lentiviral Packaging plasmids are co-transfected into an HEK293T cell line, and then viral particles are harvested, concentrated and purified to provide a high-titer preparation. All SMARTchoice shRNA particles are manufactured under stringent quality control guidelines.