How do I know if my target cell type can be transduced with SMARTchoice Lentiviral shRNA Particles?
SMARTchoice viral particles are manufactured with a VSVg envelope protein that provides broad tropism. Therefore, most cell types are transduced, albeit, with varying degrees of efficiency. However, the efficiency of gene silencing is not only a factor of successful transduction, but is also dependent upon the efficiency of transgene expression; this can be assessed by utilizing the SMARTchoice shRNA Promoter Selection Plate assay with the cells of interest. This assay will allow the simultaneous evaluation of seven SMARTchoice promoters.