How is the shRNA transcribed?
(For GIPZ) The TurboGFP, puromycin, and shRNA are all transcribed as one long transcript. The shRNA ends up curling around on itself (making the hairpin structure). Drosha comes along and cleaves the hairpin off of the transcript. The TurboGFP and puromycin are then translated (with the help of the IRES). The same principle can be applied for our other types of hairpins/vectors.