I am using human cell lines in my research; should I always choose a human promoter for best activity?
In some instances promoter activity correlates with the species from which it is derived. However, promoter activity does not always follow a species-specific expression pattern. For example, we have observed mouse promoters to be the most active in some human cell lines, whereas both human and mouse promoters were most active in some rat cells. Choosing the most effective promoter in a particular cell line is not always predictable, and therefore should be determined empirically.