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What packaging system do I need to use to produce viral particles with pGIPZ, pTRIPZ, pLEX, pLOC or pLemir?

You will need to use a 2nd generation packaging system (one that expresses the tat gene) to produce viral particles with pGIPZ, pTRIPZ, pLEX, pLOC or pLemir because it contains a wt 5' LTR which requires the binding of tat for activation. We recommend our Trans-Lentiviral Packaging System for lentivirus production with pGIPZ or pTRIPZ: http: //dharmacon. horizondiscovery. com/viral-packaging/trans-lentiviral-shrna-packaging-system/?Parent=2147483807