Can I use Dharmacon siRNA reagents in an in vivo system?
Dharmacon offers an in vivo processing option designed for siRNAs in animal models. siRNAs synthesized by 2' ACE synthesis chemistry have an amine counterion present during synthesis; therefore, we recommend and offer a salt exchange to the sodium counterion followed by dialysis to remove excess salt ions in the preparation of siRNAs intended for use in animals. Read more here about these processing options. http: //dharmacon. horizondiscovery. com/services/custom-synthesis/rna/#Purification