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How can I get more 5X siRNA Buffer?

The 5X siRNA Buffer is available for purchase on our web site. Alternatively, under RNase-free conditions, you may prepare additional 5X siRNA Buffer. Composition: 300mM KCl, 30mM HEPES-pH 7. 5, 1. 0mM MgCl2Consumables: 1. 100 mL sterile bottle or flask2. 15 mL conical tubeChemicals: 1. RNase-free water2. Potassium chloride3. HEPES-free acid4. Magnesium Chloride 6H2O5. Potassium Hydroxide pelletsProcedure: 1. Prepare annealing buffer in a sterile 100mL tubea. 30 mL RNase-free waterb. 2. 24 g potassium chloridec. 0. 72 g HEPES-free acidd. 0. 02 g magnesium chloride 6H2O2. Prepare fresh 2. 0 M KOH in a separate 15 mL conical tubea. 1. 20 g potassium hydroxide pelletsb. 10. 0 mL of RNase-free water3. Add 2. 00 mL of the freshly prepared 2. 0 M KOH to the annealing buffer from step 14. Add sterile RNase-free water and mix to a fi nal volume of 100. 0 mL5. Aliquot 1. 0 mL for pH testing. The pH should be between 7. 3-7. 66. Sterile filter or autoclave the solution7. Store the solution at 4�C for 3 months