Can HAP1 cells be used in xenografts?
Tumorigenicity of HAP1 cells in immuno-competent mice has been assessed by OncoTest. Three groups with three female NMRI nu/nu mice each received bilateral subcutaneous injections (left and right flank) of HAP1 cells (5 x 105, 1 x 106, 5 x 106 cells per injection site). During the 90-day observation phase tumors grew out at 8 of 18 injection sites in 6 out of 9 mice. Their volumes ranged from 111 mm3 to larger than 1700 mm3. The latency period for the onset of tumor growth ranged from 19 to 78 days. No deaths or progressive body weight loss of tumor-bearing mice and no clinical signs were observed.