What will I receive when I order a HAP1 knockout cell line?
Customers obtain a clonal HAP1 cell line, bearing a frameshift mutation in an exon of the gene of interest. Typically, the mutation is placed closed to the 5'end of the gene, ie. in exon 1 or 2. The mutation is described in the clone datasheet enclosed upon shipment and can be verified by a PCR on genomic DNA followed by Sanger sequencing. All mutant cell lines are provided as an isogenic pair with the wild type line. This provides a control for your downstream experiments.