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Can I use the Edit-R SAM tracrRNA with the VPR system?

No. Because of the added MS2 aptamer sequence, Edit-R SAM tracrRNA is specifically designed for use in activation experiments with cells expressing the SAM components dCas9-VP64 and MS2-p65-HSF1. If working in the dCas9-VPR system, the standard Edit-R tracrRNA (Cat# U-002005-XX) should be used for synthetic crRNA: tracrRNA experiments.