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Can I use the synthetic crRNA and tracrRNA components with other Cas9-expressing plasmids that are optimized for my biological system?

The crRNAs and tracrRNA have been tested only with the Edit-R Cas9 expression plasmid in mammalian cell lines. However, the repeat component of the crRNA and the entire tracrRNA are sequences derived from S. pyogenes, so in theory these RNAs can be used with a S. pyogenes Cas9-expressing plasmid optimized for other biological systems (with an optimal promoter and codon usage for Cas9 expression). We cannot assure or guarantee any results.