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Do I have to use Cas9:tracrRNA:crRNA with DharmaFECT Duo Transfection Reagent?

This system has been tested for use with DharmaFECT Duo, however, other reagents suitable for co-transfection of plasmid and RNA could be utilized, provided transfection optimization is performed. We cannot speak to any results obtained using other transfection reagents, nor can we provide any troubleshooting for poor transfection efficiency or cellular toxicity observed when other reagents are utilized.