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Do crRNAs synthesized using Dharmacon 2'-ACE chemistry need to be HPLC-purified for gene editing in vitro?

The proprietary Dharmacon 2'-ACE RNA synthesis chemistry has very high coupling efficiencies resulting in RNA oligos of exceptional yield and crude purity. Further crRNA purification is not necessary for gene editing experiments. Internal testing and successful gene editing were performed using desalted and deprotected crRNAs.