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When should I use siRNA, shRNA, or Edit-R to study my gene of interest?

For transient knockdown of a gene, siRNA is appropriate. For stable knockdown of a gene or where you would like to track stably-transduced cells using fluorescence, use shRNA. Both of these RNAi technologies cause knockdown of gene expression at the mRNA level. Edit-R is best for applications where a clonal line containing complete disruption and knockout of a gene at the DNA level is required.