There are no delays on our products or support as a result of COVID-19. If there is a way we can assist you, we are here to help - Contact us

Can the pCMV-SPORT6 be used with an IVT reaction?

We have not used the pCMV-SPORT6 vector in house with an IVT reaction so we cannot guarantee that it will work but the vector does have a T7 promoter (traditional promoter of use for IVT) . However, in most cases it is located downstream of the gene insert so it would not be in the correct position for an IVT reaction (it would be at the C terminal end of the ORF). There is an SP6 sequence also present in the vector and there are commercially available kits for IVT using SP6.