What concentration should I use for miRIDIAN Mimics and Hairpin Inhibitors?
Concentration curves should always be conducted to empirically determine the best concentration for each miRNA and each cell system. Suggested starting concentrations are 50 nM for the Mimic and 25 nM for the Hairpin Inhibitor when using a lipid-based delivery system such as the DharmaFECT transfection reagents. The recommended range for optimizations is 0. 4 to 100 nM.