Why do I need to use a Non-targeting control shRNA with my SMARTchoice Inducible shMIMIC microRNA?
It's a good idea to use a non-targeting control even if there is little of no shMIMIC microRNA expression in the absence of doxycyxline. Although doxycycline is generally accepted as "inert", some cellular phenotypes, such as reduced growth rate, maybe observed upon exposure to doxycycline. In addition, fluorescent reporters such as TurboGFP and TurboRFP cancause some subtle changes in cellular phenotypes. To rigorously control for both of these effects, it is necessary touse a vector-matched Non-Targeting Control shRNA to identify true mature microRNA-dependent phenotypes.