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How long does inhibition last after the transfection of miRIDIAN microRNA Inhibitors?

The duration of inhibition depends greatly on how well-expressed a mature microRNA is in a particular cell line. Inhibitors generally last longer than 48 hours in cells. The inhibitors are stabilized by their chemical modification pattern and have performed well in-house to at least 96 hours after transfection. We would suggest a time-course experiment to determine, empirically, how long they will remain potent in your system.