What was the time point for data collection in the NCI pGIPZ cancer gene validation assay?
Puromycin selection was started 48 hrs after infection and maintained 4 days for MCF-7 and 5 days for OVCAR-8, then the cell were lysed for mRNA extraction. Description and results of the validation project can be found at the following link: http: //cgap. nci. nih. gov/RNAi/shRNAValidation