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Are negative control siRNAs really necessary?

A non-targeting siRNA control is important to help establish that any decrease in gene expression levels observed with a gene-specific siRNA is related to a sequence-specific RNAi event. Global down-regulation events may be due to cellular stress responses to a certain transfection reagent or technique. Without negative controls, a researcher might mistakenly interpret this broad, non-specific silencing as true gene-specific silencing. To control for non-RNAi related or non-specific effects, Dharmacon offers a variety of products for RNAi experiments. Refer to our Recommended RNAi Control products or download our Technical Note: Effective Controls for RNAi Experiments to learn more.