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What is the benefit of using a SMARTpool siRNA reagent?

The primary benefit of using a pool of highly functional siRNAs is a reduction of off-target effects. In addition, the efficiency of a SMARTpool typically mirrors that of the most functional siRNA in the pool. Together, these benefits allow researchers to use a single reagent for efficient and specific silencing of their gene of interest. These features are especially advantageous when using siRNAs for screening purposes as they result in fewer false positives and false negatives (respectively) while reducing the amount of reagents and consumables needed compared to screening with individual siRNAs.