Gene manipulation in iPSCs

Resources, services and tools to support your iPSC research

 

Induced pluripotent stem cells (iPSCs) serve as ideal disease models due to their superior biological relevance and isogenic nature, however gene editing in iPSCs can present a unique set of experimental challenges.

Horizon's cell line engineering team has vast experience making a variety of edits in iPSCs, and here you will find our most helpful services, reagents and resources to support your iPSC research, including:

  1. Details about our expert iPSC engineering services
  2. Reagents for achieving CRISPR knockout, HDR mediated knock-in, CRISPR interference, CRISPR activation or RNAi in iPSCs
  3. Resources including application notes, posters and reading lists for gene manipulation in iPSCs
  4. Customizable screening libraries and iPSC screening services

Get started editing iPSCs

Horizon offers expert engineering services and guaranteed CRISPR reagents for gene editing in iPSCs

Why edit in iPSCs?

Engineered iPSCs often serve as superior models of difficult-to-represent diseases than traditional immortalized cell lines. Researchers edit iPSCs to create isogenic cell models containing key disease-driving mutations to achieve mechanistic understanding, without background genetic variability, of causative relationships between genotype and phenotype. 

Essentially, these edited iPSC lines serve as "disease-in-a-dish" models helping researchers to discover novel drug targets with increased predictability of their safety and efficacy.


 

iPS cell line engineering services

Many labs simply choose to outsource the engineering portion of their research and focus on downstream phenotypic analysis. Trusting your iPS cell line editing work to a dedicated genome engineering CRO can provide significant cost and time savings. 

For nearly a decade, Horizon's engineering team has successfully delivered thousands of gene editing projects for major drug discovery, biotech and pharma research. Our vast expertise working with iPSCs provides our partners with confidence that their project with be completed accurately and on time.

We currently offer genome editing services for:

  • Knockout (single or multiple)
  • Knock-in
  • Reporters

Project workflow

Once the project scope and timeline are defined, partners receive regular updates from their dedicated project manager.

iPSC engineering workflow

 

Pluripotency guarantee

We know the importance of maintaining pluripotency throughout the entire gene editing process. At Horizon we take careful measures to maintain the pluripotency status of your cells. In addition, Alkaline Phosphatase staining is provided free of charge for all our iPS cell editing projects. If you require extra assurance, we can also provide screening for additional pluripotency markers.

iPSC engineering pluripotency guarantee

 

Engineering capabilities

Knockout

Confirmation of genotype 

(A) Deletion of A nucleotide at the double strand break site leading to a premature stop codon at the target locus. 

Differentiation assessment

(B) Pluripotency and trilineage  differentiation assessment of the knockout clones. 

 

 

Knock-in

Confirmation of genotype 

(A) homozygous point mutation GAG>AAG

Confirmation of heterozygosity

(B) ddPCR assessment of the SNP/point mutation confirms the required heterozygosity

 

Reporter cell lines


 

Reagents for gene manipulation in iPSCs

Horizon offers a wide variety of reagents for use in iPSCs.

Build a custom CRISPR or siRNA library

Design your own iPSC screening library with as few as 20 individual siRNA or CRISPR guide RNA using our Cherry-Pick Library Tool. Simply upload your gene list to get started!