Top peer reviewed scientific articles using HAP1 cell lines

Can I use the HAP1 cell line for my research?

The HAP1 cell line has been applied across a wide range of biological processes, such as DNA damage repair pathway and stress responses, as well as in disease modeling. These selected articles show the broad applicability of the HAP1 cell line, and provide characterization data to help your research. If you would like to know more, please follow these links to our ready made cell lines and cell line engineering services.

Publications:

2019

Genetic interaction between DNA repair factors PAXX, XLF, XRCC4 and DNA-PKcs in human cells FEBS OpenBio
TRIM5α Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation Cell Reports
Dihydronicotinamide riboside is a potent NAD+ concentration enhancer in vitro and in vivo Journal of Biological Chemistry
A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus Nature Communications
Precise tuning of cortical contractility regulates cell shape during cytokinesis Biorxiv
Uracil–DNA glycosylase UNG1 isoform variant supports class switch recombination and repairs nuclear genomic uracil Nucleic Acid Research
Ribosomal protein RACK1 facilitates efficient translation of poliovirus and other viral IRESs Biorxiv
Integrin activation by the lipid molecule 25-hydroxycholesterol induces a proinflammatory response Nature Communications
Integrin activation by the lipid molecule 25-hydroxycholesterol induces a proinflammatory response levels and enterovirus replication Biorxiv
RanBP2/Nup358 enhances RNAi activity by sumoylating and stabilizing Argonaute 1 Biorxiv
h4A.Z-dependent and -independent recruitment of metabolic enzymes to chromatin required for histone modifications Biorxiv
Protein disulfide-isomerase A3 significantly reduces ischemia-induced damage by reducing oxidative and endoplasmic reticulum stress Neurochemistry International
Cristae undergo continuous cycles of fusion and fission in a MICOS-dependent manner Biorxiv
Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies; Cell Reports
Efficient and flexible tagging of endogenous genes by homology-independent intron targeting Genome Research
A Versatile Strategy to Reduce UGA-Selenocysteine Recoding Efficiency of the Ribosome Using CRISPR-Cas9-Viral-Like-Particles Targeting Selenocysteine-tRNA[Ser]Sec Gene Cells
Two distinct nuclear stress bodies containing different sets of RNA-binding proteins are formed with HSATIII architectural noncoding RNAs upon thermal stress exposure Biochemical and Biophysical Research Communications
CRISPR knockout screen implicates three genes in lysosome function Scientific Reports
Functional and cellular localization diversity associated with Fukutin-related protein patient genetic variants Human Mutation
A Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death Cell Chemical Biology
Bypassing pan-enterovirus host factor PLA2G16 Nature Communications
Transcriptomics of a KDELR1 knockout cell line reveals modulated cell adhesion properties Scientific Reports
Dominant TOM1 mutation associated with combined immunodeficiency and autoimmune disease Genomic Medicine
Human FAM173A is a mitochondrial lysine-specific methyltransferase that targets adenine nucleotide translocase and affects mitochondrial respiration Journal of Biological Chemistry
TMEM70 is an assembly factor of mitochondrial complexes I and V bioRxiv
Uracil–DNA glycosylase UNG1 isoform variant supports class switch recombination and repairs nuclear genomic uracil Nucleic Acid Research
The moonlighting RNA-binding activity of cytosolic serine hydroxymethyltransferase contributes to control compartmentalization of serine metabolism Nucleic Acid Research
Deoxyuridine in DNA has an inhibitory and promutagenic effect on RNA transcription by diverse RNA polymerases Nucleic Acid Research
GPN does not release lysosomal Ca2+ but evokes Ca2+ release from the ER by increasing the cytosolic pH independently of cathepsin C Journal of Cell Science
Increased MTh2-specific 8-oxodGTPase activity is a hallmark of cancer in colon, lung and pancreatic tissue DNA Repair
BCDIN3D regulates tRNAHis 3’ fragment processing PLOS Genetics
Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells; Autophagy
The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection; BioRxiv
iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture; Nature Comms
Mapping the Human Kinome in Response to DNA Damage; Cell Reports
A viral RNA motif involved in signaling the initiation of translation on non-AUG codons; RNA
Cyclodextrin triggers MCOLN1-dependent endo-lysosome secretion in Niemann-Pick type C cells; Journal of Lipid Research
PTEN self-regulates through USP11 via the PI3K-FOXO pathway to stabilize tumor suppression; Nature Comms
Deoxyuridine in DNA has an inhibitory and promutagenic effect on RNA transcription by diverse RNA polymerases; Nucleic Acid Research
GPN does not release lysosomal Ca2+ but evokes Ca2+ release from the ER by increasing the cytosolic pH independently of cathepsin C; Journal of Cell Science
DEGS1-associated aberrant sphingolipid metabolism impairs nervous system function in humans; The Journal of Clinical Investigation
Cancer mutational burden is shaped by G4 DNA, replication stress and mitochondrial dysfunction; Progress in Biophysics and Molecular Biology
The deSUMOylase SENP2 coordinates homologous recombination and nonhomologous end joining by independent mechanisms; Genes & Development
The highly GABARAP specific rat monoclonal antibody 8H5 visualizes GABARAP in immunofluorescence imaging at endogenous levels; Scientific Reports
ACBD3 Is an Essential Pan-enterovirus Host Factor That Mediates the Interaction between Viral 3A Protein and Cellular Protein PI4KB; mBio
Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh6 expression; Journal of NeuroVirology
BAP1 complex promotes transcription by opposing PRC1-mediated h4A ubiquitylation; Nature Comms
Selective pharmacological inhibitors of HDAC6 reveal biochemical activity but functional tolerance in cancer models; International Journal of Cancer
Lysine methylation by the mitochondrial methyltransferase FAM173B optimizes the function of mitochondrial ATP synthase; Journal of Biological Chemistry
Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes; PNAS
PARP1 Trapping by PARP Inhibitors Drives Cytotoxicity in Both Cancer Cells and Healthy Bone Marrow; Molecular Cancer Research
Stereoselective fatty acylation is essential for the release of lipidated WNT proteins from the acyltransferase Porcupine (PORCN); J Biological Chemistry
Myosin IIA drives membrane bleb retraction; Molecular Biology of the Cell
Multi-omics identify xanthine as a pro-survival metabolite for nematodes with mitochondrial dysfunction; EMBO Journal
A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity; Cell Reports
A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations; Scientific Reports
A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus; Nature Comms
Synthetic lethality between DNA repair factors Xlf and Paxx is rescued by inactivation of Trp53; Cell Death Discovery
h4A.Z-dependent and -independent recruitment of metabolic enzymes to chromatin required for histone modifications; BioRxiv

2018

Transcriptional and epigenetic profiling of nutrient-deprived cells to identify novel regulators of autophagy; Autophagy
IP3 Receptors Preferentially Associate with ER-Lysosome Contact Sites and Selectively Deliver Ca2+ to Lysosomes; Cell Reports
SEC24A identified as an essential mediator of thapsigargin-induced cell death in a genome-wide CRISPR/Cas9 screen; DNA Repair
Accurate classification of BRCA1 variants with saturation genome editing; Nature
Facilitated Diffusion Mechanisms in DNA Base Excision Repair and Transcriptional Activation; Chem Rev
Role of ZIP8 in regulation of cisplatin sensitivity through Bcl-2; Toxicology and Applied Pharmacology
CRISPRa-mediated NEAT1 lncRNA upregulation induces formation of intact paraspeckles; Biochemical and Biophysical Research Communications
The Importance of Poly(ADP-Ribose) Polymerase as a Sensor of Unligated Okazaki Fragments during DNA Replication; Molecular Cell
The SUMO protease SENP1 and the chromatin remodeller CHD3 interact and jointly affect chromatin accessibility and gene expression; Journal of Biological Chemistry
Validating the concept of mutational signatures with isogenic cell models; Nature Comms
Differential inhibitory effect of a pyrazolopyran compound on human serine hydroxymethyltransferase-amino acid complexes; Archives of Biochemistry and Biophysics
Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation; Mol Cell
Map of synthetic rescue interactions for the Fanconi anemia DNA repair pathway identifies USP48; Nature Comms
Isobaric Tag-Based Protein Profiling of a Nicotine-Treated Alpha7 Nicotinic Receptor-Null Human Cell Line; Proteomics
DNA damage response signaling does not trigger redistribution of SAMHD1 to nuclear foci; BBRC
Robust DNA repair in PAXX-deficient mammalian cells; FEBS
Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation; Cell Death & Differentiation
Abnormal RNA splicing and genomic instability after induction of DNMT3A mutations by CRISPR/Cas9 gene editing; Blood Cells, Molecules, and Diseases
The BAP1 deubiquitinase complex is a general transcriptional co-activator; BioRxIV

2017

A Global Interactome Map of the Dengue Virus NS1 Identifies Virus Restriction and Dependency Host Factors; Cell Reports
NRP2 and CD63 Are Host Factors for Lujo Virus Cell Entry; Cell Host Microbe
B3GALNT2 mutations associated with nonsyndromic autosomal recessive intellectual disability reveal a lack of genotype–phenotype associations in the muscular dystrophy-dystroglycanopathies; Genome Medicine
A small-molecule activator of kinesin-1 drives remodeling of the microtubule network; PNAS
Vasohibins encode tubulin detyrosinating activity; Science
Fibrinogen Activates BMP Signaling in Oligodendrocyte Progenitor Cells and Inhibits Remyelination after Vascular Damage; Neuron
TBC1D23 is a bridging factor for endosomal vesicle capture by golgins at the trans-Golg; Nature Cell Biol
Tools to Measure Autophagy Using High Content Imaging and Analysis; Methods Mol Biol
Parallel genome-wide screens identify synthetic interactions between the BLM helicase complex and Fanconi anemia; Nature Communications
Measurement of nanoscale DNA translocation by uracil DNA glycosylase in human cells; Nucleic Acids Research
CRISPR/Cas9-Mediated Scanning for Regulatory Elements Required for HPRT1 Expression via Thousands of Large, Programmed Genomic Deletions; American Society of Human Genetics
Trap^Seq: An RNA Sequencing-based pipeline for the identification of genetrap insertions in mammalian cells; J Mol Biol
Biallelic mutations in the ubiquitin ligase RFWD3 cause Fanconi anemia; J Clin Invest
RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination; Molecular Cell
eIF1 modulates the recognition of suboptimal translation initiation sites and steers gene expression via uORFs; Nucleic Acids Research
Nit1 is a metabolite repair enzyme that hydrolyzes deaminated glutathione; PNAS
FBXO25 regulates MAPKsignaling pathway through inhibition of ERK1/2 phosphorylation; Archives of Biochemistry and Biophysics
Persistence of the mitochondrial permeability transition in the absence of subunit c of human ATP synthase; PNAS
Translation of Sindbis Subgenomic mRNA is Independent of eIF2, eIF2A and eIF2D; Scientific Reports
Stress-specific differences in assembly and composition of stress granules and related foci; J Cell Sci

2016

Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling; eLife
Recycling of Apolipoprotein(a) After PlgRKT-Mediated Endocytosis of Lipoprotein(a); Circulation Research
The RNA-binding protein Secisbp2 differentially modulates UGA codon reassignment and RNA decay; Nucleic Acids Research
The Selenocysteine-Specific Elongation Factor Contains Unique Sequences That Are Required for Both Nuclear Export and Selenocysteine Incorporation; PLoS One
Dual loss of succinate dehydrogenase (SDH) and complex I activity is necessary to recapitulate the metabolic phenotype of SDH mutant tumors; Metabolic Engineering
Parallel reverse genetic screening in mutant human cells using transcriptomics; Molecular Systems Biology
TSSC1 is Novel Component of the Endosomal Retrieval Machinery; Mol Biol Cell
Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens; Nature
Opposing Roles of Double-Stranded RNA Effector Pathways and Viral Defense Proteins Revealed with CRISPR/Cas9 Knock-Out Cell Lines and Vaccinia Virus Mutants; J Virol
Chromatin regulators as a guide for cancer treatment choice; Mol Cancer Ther
ISPD produces CDP-ribitol used by FKTN and FKRP to transfer ribitol phosphate onto α-dystroglycan; Nature Communications
Direct á-synuclein promoter transactivation by the tumor suppressor p53; Mol Neurodegener

2015

Gene essentiality and synthetic lethality in haploid human cells; Science
Sialic acid-dependent cell entry of human enterovirus D68; Nature Communications
Subunit composition of VRAC channels determines substrate specificity and cellular resistance to Pt-based anti-cancer drugs; EMBO J
A forward genetic screen reveals novel independent regulators of ULBP1, an activating ligand for natural killer cells; eLife
Subunit interactions of the disease-related hexameric GlcNAc-1-phosphotransferase complex; Human Molecular Genetics
9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate; Nature Communications
Disease mutations in CMP-sialic acid transporter SLC35A1 result in abnormal α-dystroglycan O-mannosylation, independent from sialic acid; Human Molecular Gentics
KPC1-mediated ubiquitination and proteasomal processing of NF-κB1 p105 to p50 restricts tumor growth; Cell
DAG1 mutations associated with asymptomatic hyperCKemia and hypoglycosylation of a-dystroglycan; Neurology
Milder forms of muscular dystrophy associated with POMGNT2 mutations; Neurol Genet

2014

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