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CRISPRmod CRISPRa All-in-one Lentiviral sgRNA Positive Controls

Controls to verify gene activation

Validated CRISPRa All-in-one lentiviral sgRNA positive controls used to establish activity of CRISPR activation in a cell type of interest.

Dharmacon

Validated positive controls for the verification of gene activation events

CRISPRmod CRISPRa All-in-one lentiviral sgRNA positive controls should be included in all gene activation experiments using the All-in-one format to confirm successful transduction by observing the upregulation of POU5F1 or TTN genes.

Individual CRISPRa All-in-one lentiviral sgRNA positive controls are provided as concentrated, purified lentiviral particles (50 µL at ≥ 1 x 107 TU/mL); available for human and mouse and designed against:

  • POU Class 5 Homeobox 1 (POU5F1)
  • Titin (TTN)

Note: The level of activation observed will depend on your cell type and basal level of expression. If either TTN or POU5F1 is already expressed in your cells, there will be more modest activation than with a gene that is not expressed. Normalization of the activation level to a non-targeting control will provide a baseline for determining optimal dCas9-VPR expression, transfection efficiency, and timepoint for your assay.