- CRISPR interference reagents
- CRISPRi All-in-one Lentiviral sgRNA
CRISPRi All-in-one Lentiviral sgRNA
dCas9-SALL1-SDS3 and sgRNA expression from a single vector for simplified delivery
- Easy, ready-to-use (thaw virus, add to cells)
- Single transduction = shorter time to results
- Ideal for difficult to transfect cells and low passage cells
- Enrich with either puromycin resistance or EGFP fluorescence
- Transduction-ready vectors eliminate cloning and in vitro transcription steps

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Easily generate a stable cell line repressing your gene of interest with the CRISPRmod CRISPR interference (CRISPRi) system. This system is a unique adaptation of the classical CRISPR-Cas9 gene editing system which utilizes a catalytically deactivated Cas9 (dCas9) that is fused fused to repressor domains (SALL1 and SDS3). When paired with a well-designed guide RNA that targets a gene near it's promoter region or transcriptional start site (TSS) it reduces transcription.
Review our CRISPRi application page to get an overview of the technology.
CRISPR modulation all-in-one lentiviral workflow image
CRISPRi All-in-one Lentiviral sgRNA controls
CRISPRi All-in-one Lentiviral sgRNA Positive Controls
- All-in-one lentiviral sgRNAs targeting well-characterized genes to determine the effectiveness of your experimental conditions for maximum transcriptional repression.
CRISPRi All-in-one Lentiviral sgRNA Non-targeting Controls
- Bioinformatically designed All-in-one Non-targeting sgRNA controls to evaluate baseline cellular responses to CRISPRi components in the absence of target-specific sgRNA.
Transcriptional Repression with CRISPRmod All-in-one Puro-dCas9-SALL1-SDS3
Selection markedly improves transcriptional repression in U2OS cells
Product inserts
Safety data sheets
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All-in-one non-targeting constructs are bioinformatically designed and validated to not target any gene in human or mouse genomes.
Validated CRISPRmod CRISPRi All-in-one lentiviral sgRNA positive controls used to establish activity of CRISPR interference in a cell type of interest.