CRISPRmod CRISPRi All-in-one Lentiviral sgRNA
dCas9-SALL1-SDS3 and sgRNA expression from a single vector for simplified delivery in human
- Easy, ready-to-use lentiviral particles (thaw virus, add to cells)
- Single transduction = shorter time to results
- Ideal for difficult to transfect cells and low passage cells
- Enrich with either puromycin resistance or EGFP fluorescence
- Transduction-ready vectors eliminate cloning and in vitro transcription steps
1Start Here
Easily generate a stable cell line repressing your gene of interest with the CRISPRmod CRISPR interference (CRISPRi) system. This system is a unique adaptation of the classical CRISPR-Cas9 gene editing system which utilizes a catalytically deactivated Cas9 (dCas9) that is fused to repressor domains (SALL1 and SDS3). When paired with a well-designed guide RNA that targets a gene near its promoter region or transcriptional start site (TSS) it reduces transcription.
The choice of a puromycin resistance gene or EGFP marker allows for selection of cells that have successfully integrated the vector. The EGFP selection marker is recommended for rapid enrichment of edited cells, as FACS analysis may be performed as soon as fluorescence is expressed. This is especially useful for short-lived cell types, such as primary cells.
The predesigned All-in-one CRISPRi Lentiviral sgRNAs are available as:
- High quality, concentrated, purified lentiviral particles for direct transduction with minimal cytotoxicity; delivered at titers of ≥ 1 x 107 TU/mL
- Designs optimized from a published algorithm by Horlbeck, et. al. demonstrate strong levels of gene repression (see References tab)
- For genes with alternative characterized start sites, distinct guide RNA designs are available (labeled P2).
Review our CRISPRi application page and the AIO application page to get an overview of the technology.
