Easily generate a stable cell line repressing your gene of interest with the CRISPRmod CRISPR interference (CRISPRi) system. This system is a unique adaptation of the classical CRISPR-Cas9 gene editing system which utilizes a catalytically deactivated Cas9 (dCas9) that is fused to repressor domains (SALL1 and SDS3). When paired with a well-designed guide RNA that targets a gene near its promoter region or transcriptional start site (TSS) it reduces transcription.
The choice of a puromycin resistance gene or EGFP marker allows for selection of cells that have successfully integrated the vector. The EGFP selection marker is recommended for rapid enrichment of edited cells, as FACS analysis may be performed as soon as fluorescence is expressed. This is especially useful for short-lived cell types, such as primary cells.
The predesigned All-in-one CRISPRi Lentiviral sgRNAs are available as:
All-in-one non-targeting constructs are bioinformatically designed and validated to not target any gene in human or mouse genomes.
Validated CRISPRmod CRISPRi All-in-one lentiviral sgRNA positive controls used to establish activity of CRISPR interference in a cell type of interest.