CRISPRi All-in-one Lentiviral sgRNA

dCas9-SALL1-SDS3 and sgRNA expression from a single vector for simplified delivery

  • Easy, ready-to-use (thaw virus, add to cells)
  • Single transduction = shorter time to results
  • Ideal for difficult to transfect cells and low passage cells
  • Enrich with either puromycin resistance or EGFP fluorescence
  • Transduction-ready vectors eliminate cloning and in vitro transcription steps
Dharmacon
To shop for CRISPRi All-in-one Lentiviral sgRNA, please select a Gene.

1Start Here

crispri-all-in-one-vector-map

Easily generate a stable cell line repressing your gene of interest with the CRISPRmod CRISPR interference (CRISPRi) system. This system is a unique adaptation of the classical CRISPR-Cas9 gene editing system which utilizes a catalytically deactivated Cas9 (dCas9) that is fused fused to repressor domains (SALL1 and SDS3). When paired with a well-designed guide RNA that targets a gene near it's promoter region or transcriptional start site (TSS) it reduces transcription.

Review our CRISPRi application page to get an overview of the technology.

Related Products

CRISPRi All-in-one Lentiviral sgRNA Non-targeting Controls

All-in-one non-targeting constructs are bioinformatically designed and validated to not target any gene in human or mouse genomes.

These non-targeting controls should be used to establish experimental baselines and to distinguish sequence-specific biological effects from non-specific effects.

View Options
CRISPRmod CRISPRi All-in-one Lentiviral sgRNA Positive Controls

Controls to verify gene repression (or transcriptional repression) 

Validated CRISPRmod CRISPRi All-in-one lentiviral sgRNA positive controls used to establish activity of CRISPR interference in a cell type of interest.

View Options