Preclinical scale and OEM RNA and DNA synthesis

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Quality custom oligonucleotides for research and preclinical applications

Our preclinical/OEM manufacturing service provides our academic and industry partners with cost-effective and timely oligonucleotide materials for novel research (in vitro) thru preclinical use (in vivo).

Oligo manufacturing from Dharmacon offers:

  • An ISO 9001-certified manufacturing facility and a highly trained and dedicated custom oligonucleotide synthesis team
  • Preclinical scale synthesis (50 mg to multiple grams) of both RNA and DNA oligonucleotides synthesized by our proprietary 2’-ACE or traditional 2’-TBDMS chemistry
  • An extensive selection of chemical modifications such as modified nucleobases, phosphorothioates, linkers, spacers, and fluorescent dyes
  • Customization and contract production, including OEM oligonucleotide reagents to meet customer needs

Are you interested in a preclinical/OEM custom service? To get started, please request pricing with our custom oligonucleotide synthesis form. For further assistance, please contact Technical Support

Purified 42-mer crRNA
analysis 42 mer oligo anion exchange hplc 
analysis 42 mer oligo mass 

Purity analysis and characterization of a 42-mer crRNA oligonucleotide. The 42-mer RNA was synthesized using 2’-O-TBDMS chemistry at 120 µmol scale. After cleavage and deprotection, the RNA oligonucleotide was purified by anion-exchange chromatography. Pooled fractions were desalted by ultra-filtration followed by freeze drying to yield lyophilized RNA. (A) Analytical ion-pairing reverse phase UPLC trace showing 92% full-length product (FLP); (B) Analytical anion-exchange HPLC trace showing 89% full-length product (FLP); (C) Mass was analyzed with the LXQ Mass Spectrometer, confirming the correct mass.

Purified 30-mer DNA
analysis 30 mer oligo 

analysis 30 mer oligo mass

Analysis of a 30-mer DNA oligonucleotide with final delivery yield of 1 g. (A) Analytical trace from ion pairing reverse phase UPLC showing 97% full length material. (B) Mass was analyzed by ESI-MS, results illustrate the correct mass.



Brochures & flyers

Custom synthesis
Dharmacon custom RNA synthesis enables additional experimental abilities and scientific discoveries through unique chemistry.

Preclinical oligo
Product flyer giving an overview of the the preclinical oligo offering.


Featured articles

A role for modified synthetic RNA oligos in structural studies
Dharmacon's custom RNA synthesis provides a convenient option for your structural experiments.

Custom RNA synthesis resources
We've made it easier to understand and choose modifications for siRNA, ssRNA, 5', 3' & internal bases, and labels and dyes!

Custom RNA you can SEE
Cy Dye RNA labeling enables you to track your RNA

Our patented 2'-ACE chemistry enables advanced RNA synthesis
Longest RNA molecules synthetically possible with virtually any chemical modification!


Product inserts

Option A1 custom siRNA synthesis
Option A1 custom siRNA synthesis product insert, containing contents, protocol, shipping and storage conditions, handling precautions, etc.

Option A4 custom siRNA synthesis
Option A4 custom siRNA synthesis product insert, containing contents, protocol, shipping and storage conditions, handling precautions, etc.

Option in vivo and in vivo HPLC custom siRNA synthesis
Dharmacon option in vivo: The siRNA is shipped as a lyophilized pellet in the 2'- deprotected and duplexed form.


Deprotection of 2'-ACE protected RNA
How to deprotect 2'-ACE protected RNA

Ethanol precipitation of RNA oligonucleotides
This protocol is for Ethanol Precipitation of RNA Oligonucleotides.

Receiving RNA
Protocol for deprotecting custom synthesized RNA oligos.

Reverse phase cartridge desalting of RNA oligonucleotides
Protocol for desalting RNA oligos using reverse phase cartridges, including protocol for cartridge equilibration, washing, and eluting from cartridge.

TCEP reaction for thiol-modified siRNA/RNA oligonucleotides
Protocol to deprotect (reduce) oxidizied thiol-modified siRNA and RNA oligonucleotides.

Recommended reading

Dharmacon publications
These publications are from Dharmacon scientists highlighting internal research and collaborations in the fields of CRISPR-Cas9, RNAi, et al.

Safety data sheets

SDS (US) RNA oligonucleotides
North American standard safety data sheet for RNA oligonucleotides. 

SDS (US) deprotection buffer
North American standard safety data sheet for deprotection buffer.

SDS (EU) RNA oligonucleotides
European Union standard safety data sheet for RNA oligonucleotides. 

SDS (EU) deprotection buffer
European Union standard safety data sheet for deprotection buffer.