Oligo manufacturing from Dharmacon offers:
- An ISO 9001-certified manufacturing facility and a highly trained and dedicated custom oligonucleotide synthesis team
- Preclinical scale synthesis (50 mg to multiple grams) of both RNA and DNA oligonucleotides synthesized by our proprietary 2’-ACE or traditional 2’-TBDMS chemistry
- An extensive selection of chemical modifications such as modified nucleobases, phosphorothioates, linkers, spacers, and fluorescent dyes
- Customization and contract production, including OEM oligonucleotide reagents to meet customer needs
Are you interested in a preclinical/OEM custom service? To get started, please request pricing with our custom oligonucleotide synthesis form. For further assistance, please contact Scientific Support
Brochures & flyers
Custom synthesis
Dharmacon custom RNA synthesis enables additional experimental abilities and scientific discoveries through unique chemistry.
Featured articles
A role for modified synthetic RNA oligos in structural studies
Dharmacon's custom RNA synthesis provides a convenient option for your structural experiments.
Custom RNA synthesis resources
We've made it easier to understand and choose modifications for siRNA, ssRNA, 5', 3' & internal bases, and labels and dyes!
Custom RNA you can SEE
Cy Dye RNA labeling enables you to track your RNA
Our patented 2'-ACE chemistry enables advanced RNA synthesis
Longest RNA molecules synthetically possible with virtually any chemical modification!
Product inserts
Option A1 custom siRNA synthesis
Option A1 custom siRNA synthesis product insert, containing contents, protocol, shipping and storage conditions, handling precautions, etc.
Option A4 custom siRNA synthesis
Option A4 custom siRNA synthesis product insert, containing contents, protocol, shipping and storage conditions, handling precautions, etc.
Option in vivo and in vivo HPLC custom siRNA synthesis
Dharmacon option in vivo: The siRNA is shipped as a lyophilized pellet in the 2'- deprotected and duplexed form.
Protocols
Deprotection of 2'-ACE protected RNA
How to deprotect 2'-ACE protected RNA
Ethanol precipitation of RNA oligonucleotides
This protocol is for Ethanol Precipitation of RNA Oligonucleotides.
Receiving RNA
Protocol for deprotecting custom synthesized RNA oligos.
Reverse phase cartridge desalting of RNA oligonucleotides
Protocol for desalting RNA oligos using reverse phase cartridges, including protocol for cartridge equilibration, washing, and eluting from cartridge.
TCEP reaction for thiol-modified siRNA/RNA oligonucleotides
Protocol to deprotect (reduce) oxidizied thiol-modified siRNA and RNA oligonucleotides.
Recommended reading
Dharmacon publications
These publications are from Dharmacon scientists highlighting internal research and collaborations in the fields of CRISPR-Cas9, RNAi, et al.
Safety data sheets
SDS (US) RNA oligonucleotides
North American standard safety data sheet for RNA oligonucleotides.
SDS (US) deprotection buffer
North American standard safety data sheet for deprotection buffer.
SDS (EU) RNA oligonucleotides
European Union standard safety data sheet for RNA oligonucleotides.
SDS (EU) deprotection buffer
European Union standard safety data sheet for deprotection buffer.
