Why are HAP1 cells an ideal option for gene-edited cell line models?
Our HAP1 cell line database represents the synergy of two great systems - the amenability of human haploid HAP1s to gene editing and CRISPR-Cas9 technology. HAP1 is a human near-haploid cell line derived from the chronic myelogenous leukemia (CML) cell line KBM-7. KBM-7 cells were derived from a male patient and lack a Y chromosome. Therefore, haploid HAP1 cells contain a single X chromosome. For more details on how the HAP1 cell line was created and their full genotype, please see the references tab.
HAP1 cell line properties include:
- Sustained growth at single cell dilutions
- Ease of transfection
- Rapid doubling time
The majority of commonly used cell lines are diploid or even have more than two copies of any particular allele. When gene editing for loss of function mutations, each allele has to be modified for the phenotype to be expressed. The HAP1 cell line has the advantage of having one copy of each gene meaning you can be sure the edited allele will not be masked by addition alleles. With passaging, HAP1 cells will spontaneously diploidize over time. However, the knockout will not be affected by diploidization as it is a duplication of the edited allele that gives rise to the diploid state.
See our HAP1 publication list to see how the cell line has been applied for a wide range of biological processes and assays, such as DNA damage repair pathway, stress responses, disease modeling as well as antibody validation.
Parental cell line details
Human HAP1 Parental Cell Line
(Cat # C631) Wild-type HAP1 cells NOT SELECTED for ploidy status. C631 parental HAP1 cells are the parental cell line for all of our HAP1 knockout cell lines. It is what we use to generate the knockout cell lines, and it is also what we send along with all KO cell lines as the parental control.
Human HAP1 parental cell line (screening ready)
(Cat # C859) Quality-controlled near-haploid HAP1 wild-type cells SELECTED for ploidy status.
Human KBM-7 cell line
(Cat # C628) Quality-controlled near-haploid KBM-7 wild-type cells. All wild-type HAP1 cells were derived from the KBM-7 cell line.
Human eHAP cell line
(Cat # C669) Quality-controlled fully-haploid engineered HAP1 (eHAP) wild-type cells eHAP cells are completely haploid. We have used genome engineering to remove the fragment of chromosome 15 from chromosome 19, so no genes are duplicated in this cell line. This cell line does still have the Philadelphia chromosome.
Limited use agreement
All cell lines engineered by Horizon Discovery are purchased under the terms of our Limited Use Label License.
For more information about how the HAP1 cell line was generated, see:
- Kotecki, M., Reddy, P. S. & Cochran, B. Isolation and Characterization of a Near-Haploid Human Cell Line. Experimental Cell Research 252, 273–280 (1999). DOI: 10.1006/excr.1999.4656
- Carette, J. E. et al. Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens. Science 326, 1231–1235 (2009). DOI: 10.1126/science.1178955
- Carette, J. E. et al. Ebola virus entry requires the cholesterol transporter Niemann–Pick C1. Nature 477, 340–343 (2011). DOI: 10.1038/nature10348
- Essletzbichler, P. et al. Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line. Genome Res 24, 2059–2065 (2014). DOI: 10.1101/gr.177220.114
- Dong, M. et al. DAG1 mutations associated with asymptomatic hyperCKemia and hypoglycosylation of α-dystroglycan. Neurology 84, 273–279 (2015). DOI: 10.1212/WNL.0000000000001162
- Kravtsova-Ivantsiv, Y. et al. KPC1-mediated ubiquitination and proteasomal processing of NF-κB1 p105 to p50 restricts tumor growth. Cell 161, 333–347 (2015). DOI: 10.1016/j.cell.2015.03.001
- Lackner, D. H. et al. A generic strategy for CRISPR-Cas9-mediated gene tagging. Nat Commun 6, 10237 (2015). DOI: 10.1038/ncomms10237
For information on the KBM-7 cell line, see:
- Andersson, B. S. et al. Ph-positive chronic myeloid leukemia with near-haploid conversion in vivo and establishment of a continuously growing cell line with similar cytogenetic pattern. Cancer Genet Cytogenet 24, 335–343 (1987). DOI: 10.1016/0165-4608(87)90116-6