Our HAP1 cell line database represents the synergy of two great technologies - the amenability of human haploid HAP1s to gene editing and CRISPR-Cas9 technology.
Haploid cells have a single copy of almost every human chromosome. HAP1 is a human near-haploid cell line derived from the male chronic myelogenous leukemia (CML) cell line KBM-7. For more details on how the HAP1 cell line was created, please see the references tab.
HAP1 cell line properties include:
- Sustained growth at single cell dilutions
- Ease of transfection
- Rapid doubling time
- Haploid background
Why use a Haploid cell line?
The majority of commonly used cell lines are diploid or even have more than two copies of any particular allele. When gene editing for loss of function mutations, each allele has to be modified for the phenotype to be expressed. The HAP1 cell line has the advantage of having one copy of each gene meaning you can be sure the edited allele will not be masked by addition alleles. This is is why haploid cells have been an established tool for genetic analysis for decades, as only one allele requires modification.
Shipping conditions: Dry ice
Storage conditions: Liquid nitrogen
Biological safety level: BSL-1
How was the HAP1 cell line created? Check out these great articles below to learn more about how the HAP1 cell line was generated.
- Essletzbichler P. et al., Genome Res. 2014
Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line
- Dong M. et al., Neurology 2014
DAG1 mutations associated with asymptomatic hyperCKemia and hypoglycosylation of a-dystroglycan
- Kravtsova-Ivantsiv Y. et al., Cell 2015
KPC1-mediated ubiquitination and proteasomal processing of NF-κB1 p105 to p50 restricts tumor growth
- Carette et al. Nature. 2011
Ebola virus entry requires the cholesterol transporter Niemann–Pick C1
- Lackner DH et al. Nat Commun
A generic strategy for CRISPR-Cas9-mediated gene tagging
- Kotecki et al. Exp Cell Res. 1999
Isolation and Characterization of a Near-Haploid Human Cell Line
- Carette et al. Science. 2009
Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens