Haploid Cells

Our HAP1 cell line database represents the synergy of two great technologies - the amenability of human haploid HAP1s to gene editing and CRISPR-Cas9 technology. Haploid cells have a single copy of almost every human chromosome. HAP1 is a human near-haploid cell line derived from the chronic myelogenous leukemia (CML) cell line KBM-7. KBM-7 cells were derived from a male patient and lack a Y chromosome. Therefore, haploid HAP1 cells contain a single X chromosome. For more details on how the HAP1 cell line was created and their full genotype, please see the references tab.

HAP1 cell line properties include:

• Sustained growth at single cell dilutions
• Ease of transfection
• Rapid doubling time
• Haploid background

Why use a Haploid cell line?

The majority of commonly used cell lines are diploid or even have more than two copies of any particular allele. When gene editing for loss of function mutations, each allele has to be modified for the phenotype to be expressed. The HAP1 cell line has the advantage of having one copy of each gene meaning you can be sure the edited allele will not be masked by additional alleles. This is is why haploid cells have been an established tool for genetic analysis for decades, as only one allele requires modification.

Parental cell line details

Human HAP1 Parental Cell Line

(Cat # C631) Wild-type HAP1 cells NOT SELECTED for ploidy status. C631 parental HAP1 cells are the parental cell line for all of our HAP1 knockout cell lines. It is what we use to generate the knockout cell lines, and it is also what we send along with all KO cell lines as the parental control.

Human HAP1 parental cell line (screening ready)

(Cat # C859) Quality-controlled near-haploid HAP1 wild-type cells SELECTED for ploidy status.

Human KBM-7 cell line

(Cat # C628) Quality-controlled near-haploid KBM-7 wild-type cells. All wild-type HAP1 cells were derived from the KBM-7 cell line.

Human eHAP cell line

(Cat # C669) Quality-controlled fully-haploid engineered HAP1 (eHAP) wild-type cells eHAP cells are completely haploid. We have used genome engineering to remove the fragment of chromosome 15 from chromosome 19, so no genes are duplicated in this cell line. This cell line does still have the Philadelphia chromosome.

Limited use agreement

All cell lines engineered by Horizon Discovery are purchased under the terms of our Limited Use Label License.