HAP1 parental cell lines
Confirm your results with matched parental HAP1 cell lines
Haploid Cells
Our HAP1 cell line database represents the synergy of two great technologies - the amenability of human haploid HAP1s to gene editing and CRISPR-Cas9 technology.
Haploid cells have a single copy of almost every human chromosome. HAP1 is a human near-haploid cell line derived from the male chronic myelogenous leukemia (CML) cell line KBM-7. For more details on how the HAP1 cell line was created, please see the references tab.
HAP1 cell line properties include:
- Sustained growth at single cell dilutions
- Ease of transfection
- Rapid doubling time
- Haploid background
Why use a Haploid cell line?
The majority of commonly used cell lines are diploid or even have more than two copies of any particular allele. When gene editing for loss of function mutations, each allele has to be modified for the phenotype to be expressed. The HAP1 cell line has the advantage of having one copy of each gene meaning you can be sure the edited allele will not be masked by addition alleles. This is is why haploid cells have been an established tool for genetic analysis for decades, as only one allele requires modification.