The ASCB | EMBO 2018 Meeting will focus on cell biology as the fundamental basis of biology and explore more specialized fields, such as neurobiology and stem cell biology.
You can meet the Horizon team on Stand 710, we're looking forward to hearing about your research.
Horizon will be hosting a Tech Talk on Monday Dec 10 from 3pm-4pm.
Theater 1, Learning Center
Cell line engineering with CRISPR-Cas9 – tips and tricks to maximize success
Presenter: Vipat Raksakulthai , Field Application Specialist, Horizon Discovery
CRISPR-Cas9 is a versatile tool to discover more about your biological pathway or gene of interest. The simplicity of the CRISPR-Cas9 system has led to an explosion in applications, from single cell genomics to personalized medicine. In this Tech Talk, we will discuss key considerations when embarking on your gene engineering project to help minimize the bench time needed to reach your gene-engineering goals. Topics will range from designing guide RNA reagents to target your gene of interest to validating the resulting clones. We will also discuss the benefits of using an independent cell line model for confirmation of the phenotype. Key topics will include: Introduction to CRISPR-Cas9 genome engineering and Important considerations when initiating your gene engineering projects, including: Cell line optimization, Guide RNA selection, Donor design for knock-in studies, and Clone screening strategies and validation.
We will also be holding a drinks event on Monday Dec 10.
Venue: Mezè Greek Fusion, San Diego
We look forward to seeing you there!