What will I receive when I order a HAP1 knockout cell line?

You will receive a clonal HAP1 cell line, with a frameshift mutation in an exon of the gene of interest. Typically, the mutation is made closed to the 5' end of the gene, often in exon 1 or 2. The mutation is described in the clone datasheet (found on the cell line specific webpage) and can be verified by a PCR on genomic DNA, followed by Sanger sequencing. All mutant cell lines are provided as an isogenic pair with the wild type line as a control for your downstream experiments.