Are there different efficacies for shRNA that target the 3'UTR of a gene vs. the CDS?

While none of the shRNA are individually validated, we have anecdotal evidence to suggest that the loaded RISC seems to target as well in the 3´ UTR (although not the 5´ UTR) as in the CDS and therefore there is no particular known reason to prefer targeting the 3'UTR or the CDS. What works in one cell line may not translate to another. We offer functional guarantee for most of the shRNA products we offer, please check the product-specific page for more details.