How do I calculate how much tracrRNA I will need for my crRNA library?

Since crRNA and tracrRNA are used in equimolar amounts, be sure to purchase enough tracrRNA to complete experiments with all gene-targeting and control crRNAs. For example: For 0.1 nmol crRNA library: 0.1 nmol x 100 genes = 10 nmols of tracrRNA For 0.25 nmol library: 0.25 nmol x 100 genes = 25 nmols of tracrRNA For 0.5 nmol library: 0.5 nmol x 100 genes = 50 nmols of tracrRNA