Edit-R synthetic sgRNA non-targeting controls are recommended as negative controls for experiments using synthetic sgRNA. All Edit-R non-targeting controls are designed to have a minimum of three mismatches or gaps to all potential PAM-adjacent targets in the human or mouse genomes. Changes in viability or gene expression levels in cells treated with these controls likely reflect a baseline cellular response that can be compared to the levels in cells treated with target-specific sgRNAs.
- Proprietary alignment tools used to verify at least three mismatches or gaps to any potential target in the human or mouse genomes
- Five different designs to choose from, ensuring your chance of finding one that has no detectable effects in your system