The Luciferase Duplex is an siRNA control to effectively knock down the Photinus pyralis (firefly) luciferase gene, a popular reporter gene in many expression plasmid systems.
Because the fireflyluciferase reporter sequence is often altered to improve its performance, we recommend aligning the target siRNA sequence to your reporter to ensure a sequence match.
- Target Sequence:
- Appropriate for use in any mammalian cell type
- J. Capodici et al., Inhibition of HIV-1 Infection by Small Interfering RNA-Mediated RNA Interference. Journal of Immunology. 169(9), 5196-5201 (1 November 2002).
Concentrated buffer solution recommended for resuspension and long-term storage of any short, double-strand, or single-strand synthetic RNA molecule. Dilute with RNase-free water prior to use.
Molecular grade water for dilution of 5x siRNA Buffer or resuspension of RNA. RNase-free to prevent degradation of RNA reagents and oligonucleotides.
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments