siSTABLE Cyclophilin B Control siRNA is a validated positive control, guaranteed to silence cyclophilin B in human cells. This control siRNA is chemically modified to significantly extend siRNA stability and is recommended for use as a positive control in experiments using siSTABLE-modified siRNA against a target gene or where increased stability of the siRNA is desired.
Also known as peptidylprolyl isomerase B (PPIB), cyclophilin B is associated with the secretory pathway. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability. Targets accession number NM_000942.
- Experimentally validated for consistent, potent silencing of cyclophilin B in human cells
- Chemically modified to significantly extend siRNA stability
- Suitable for use in experimental settings with high nuclease activity
Stability of siSTABLE siRNA in 100% Human Serum
The siSTABLE modification pattern dramatically extends the half-life of siRNA in the presence of 100% human serum. Unmodified siRNA degrades almost immediately while siSTABLE siRNA maintains integrity for up to 5 days.
Potent Silencing by siSTABLE Cyclophilin B siRNA
Western analysis of Cyclophilin B protein in HeLa cells was performed 96 and 120 hours after transfection with 100 nM of the indicated siRNAs: siSTABLE Non-Targeting siRNA #1 (D-001700-01, Lanes 1 and 4), siGENOME Cyclophilin B siRNA (D-001136-01, Lanes 2 and 5), and siSTABLE Cyclophilin B siRNA (D-001710-02, Lanes 3 and 6). Transfections were performed in 96-well plates with 5 x 103 cells/well and 0.2 μL DharmaFECT 1/well.
Concentrated buffer solution recommended for resuspension and long-term storage of any short, double-strand, or single-strand synthetic RNA molecule. Dilute with RNase-free water prior to use.
Molecular grade water for dilution of 5x siRNA Buffer or resuspension of RNA. RNase-free to prevent degradation of RNA reagents and oligonucleotides.