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DharmaFECT kb DNA transfection reagent
A unique transfection reagent from our DharmaFECT product line for introduction of DNA into cultured cells with high efficiency and low cytotoxicity
Updated shipping conditions: Horizon Discovery always strives to reduce its environmental impact. Sustainability goals factor in many decisions regarding our business. By eliminating Styrofoam from North America and Canada DharmaFECT packaging, Horizon will divert >110k cubic feet of long-decomposing material from landfills every year (that’s over 33 semi-trucks), and product efficacy remains guaranteed.
DharmaFECT transfection reagents have proven to be extremely valuable tools for introducing small RNAs into cultured cells. DharmaFECT kb has the same reliable efficiency and low of toxicity as our RNA-specific reagents, but is specifically engineered for delivery of plasmids to your cells of interest.
High transfection efficiency of plasmid DNA into a variety of cell types, including: HeLa, HEK293T, U2OS, and MCF-7
Large dynamic range with low toxicity
Works well for co-transfection of Cas9 nuclease and sgRNA plasmids
Simple and familiar protocol
Works equally well in the presence or absence of serum
Successful CRISPR-Cas9 gene editing utilizing co-delivery of Edit-R Lentiviral sgRNA and Cas9 expression plasmid using DharmaFECT kb transfection reagent
U2OS cells were transfected with equal amounts of Edit-R hCMV-mKate2-Cas9 Expression plasmid (Cat #U-004100-120) and Edit-R Human PPIB Lentiviral sgRNA plasmid in a 96-well format. Transient transfections were done with increasing amounts of total DNA (50 to 200 ng) and DharmaFECT kb transfection reagent (Cat #T-2006-01), 0.2 to 0.8 µL per well. The percentage of gene editing was estimated 72 hours after transfection by DNA mismatch detection assay using T7EI with gel densitometry.
TurboGFP expression from SMARTvector lentiviral shRNA plasmid as an indicator of successful delivery with DharmaFECT kb transfection reagent
HEK293T cells were transfected with 1 µg SMARTvector Human AKT1 Lentiviral shRNA plasmid (Cat #V3SH11240-224972267) in a 24-well format. Transient transfections were done with DharmaFECT kb transfection reagent (Cat #T-2006-01) at 3 µL per well. Images were taken 48 hours after transfection.