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Overexpress genes from Yeast, C. elegans, E. coli, Zebrafish or Xenopus laevis.
The Precision LentiORF collection provides a comprehensive set of ready-to-use clones that permit RNAi rescue in studies that utilize 3'-UTR siRNA reagents. This study highlights the necessary controls and optimization required for an RNAi rescue experiment and demonstrates the utility of these constructs when validating phenotypes associated with siRNA-mediated silencing.