T cell CRISPR screens

Pooled and arrayed primary T cell screening services.
Editing primary T cells to improve T cell immunotherapies.

Pooled screens are effective for analyzing impacts of gene loss at a population level. Arrayed screens allow analyses of individual genes on a well-by-well basis and are more applicable to complex phenotypic endpoints - methods for identifying novel potential hits, which could translate into therapeutic targets.

What our service offers

  • Lentiviral sgRNA libraries designed for whole genome, gene family subsets, or custom gene list screen
  • High complexity screens are possible, up to 8 guides per gene; lower complexity can be run as project requires
  • Deep understanding of donor variability
  • Custom synthesized pools
  • Single-cell RNA-Seq linked CRISPR screening
  • Guide options available on demand
  • - Vector based sgRNA

    - Synthetic crRNA-tracrRNA

    - Synthetic sgRNA

What we deliver

Consultation with specialist team

  • Summary of screen aims
  • Key findings & conclusions
  • Tables and graphs of key data metrics
  • Data reports include raw data and quality metrics
  • Analysed data with hit calling

 

Functional genomic screening services

From target selection to patient stratification.

Identify and characterize therapeutic targets and biomarkers.

Trusted by 11 of Top 20 Pharma*

*https://www.biospace.com/article/top-20-pharma-companies-by-market-cap-in-q1-2019/

Immune cell-based assays

Understand mechanism of action, test biosimilars or identify biomarkers.

Fully compatible with any CRISPR & RNAi screening project.

Pooled vs arrayed T cell screens

CRISPR screening in primary human T cells - extending cell type capabilities

CRISPR screening in primary human T cells - extending cell type capabilities

Featured article - Pooled T-cell screens

Read our article to learn about Lentiviral sgRNA CRISPR screening in primary human immune cells.

Poster - Screening in primary immune cells

Take a look at our poster presented at AACR 2019 entitled:

RNA-based screens in primary human immune cells

Poster - Pooled and arrayed CRISPR-Cas9 knockout screens in primary human immune cells

Learn more how pooled and arrayed CRISPR screens address different questions.